BDNF antibody (AA 183-194)

Catalog No. ABIN462499

Product Details anti-BDNF Antibody

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Binding Specificity: AA 183-194

Reactivity: Human, Rat, Mouse, Pig, Horse, Dog, Guinea Pig, Cow, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BDNF. This antibody is expected to recognise all reported isoforms (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1)

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Dolphin, Hamster, Elephant, Panda, Bovine, Dog, Cat, Horse, Pig, Guinea pig, Trout, Seabass, Stickleback (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide ETKCNPMGYTKE from an internal region of human BDNF (NP_001700.2, NP_001700.2, NP_733928.1, NP_733929.1, NP_733930.1, NP_733931.1). Percent identity by BLAST analysis: Human, Chimpanzee, Monkey, Mouse, Rat, Shrew, Dolphin, Hamster, Elephant, Panda, Bovine, Dog, Cat, Horse, Pig, Guinea pig, Trout, Seabass, Stickleback (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF (BDNF Products)

Synonyms: ANON2 antibody, BULN2 antibody, bdnf-A antibody, BDNF antibody, bdnf antibody, brain derived neurotrophic factor antibody, brain-derived neurotrophic factor antibody, brain-derived neurotrophic factor L homeolog antibody, brain-derived neurotrophic factor S homeolog antibody, BDNF antibody, Bdnf antibody, bdnf antibody, bdnf.L antibody, bdnf.S antibody

Background: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

Gene ID: 627

NCBI Accession: NP_001700, NP_733928, NP_733929, NP_733930, NP_733931

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity