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WT1 antibody (N-Term)

WT1 Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN6740526
  • Target See all WT1 Antibodies
    WT1 (Wilms Tumor 1 (WT1))
    Binding Specificity
    • 30
    • 16
    • 15
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 144
    • 69
    • 62
    • 17
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Host
    • 83
    • 60
    • 1
    Rabbit
    Clonality
    • 83
    • 61
    Polyclonal
    Conjugate
    • 70
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This WT1 antibody is un-conjugated
    Application
    • 87
    • 80
    • 61
    • 55
    • 33
    • 22
    • 18
    • 14
    • 13
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    Human WT1
    Predicted Reactivity
    Percent identity by BLAST analysis: Human (100%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic peptide from N-Terminus of human WT1 (NP_000369). Percent identity by BLAST analysis: Human (100%), Gorilla, Marmoset (92%).

    Type of Immunogen: Synthetic peptide
    Isotype
    IgG
    Top Product
    Discover our top product WT1 Primary Antibody
  • Application Notes
    Approved: WB (0.2 - 1 μg/mL)

    Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

    Not recommended for: IHC-P
    Comment

    Target Species of Antibody: Human

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Distilled water
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handling Advice
    Avoid repeat freeze-thaw cycles.
    Storage
    4 °C,-20 °C
    Storage Comment
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    WT1 (Wilms Tumor 1 (WT1))
    Alternative Name
    WT1 / Wilms Tumor 1 (WT1 Products)
    Synonyms
    AWT1 antibody, EWS-WT1 antibody, GUD antibody, NPHS4 antibody, WAGR antibody, WIT-2 antibody, WT33 antibody, CWT1 antibody, WT1 antibody, XWT1a antibody, wt-1 antibody, wt1 antibody, xWT1 antibody, WT-1 antibody, gud antibody, wagr antibody, wt33 antibody, xwt1 antibody, wit-2 antibody, si:ch211-160j6.3 antibody, zgc:152697 antibody, D630046I19Rik antibody, Wt-1 antibody, XWT1b antibody, XeWT1 antibody, Wilms tumor 1 antibody, Wilms tumor 1 S homeolog antibody, wilms tumor 1b antibody, Wilms tumor protein antibody, Wilms tumor 1 homolog antibody, Wilms tumor 1 L homeolog antibody, WT1 antibody, wt1.S antibody, wt1 antibody, wt1b antibody, Wt1 antibody, wt1.L antibody
    Background
    Name/Gene ID: WT1

    Synonyms: WT1, GUD, EWS-WT1, Amino-terminal domain of EWS, WAGR, WIT-2, Wilms tumor protein, AWT1, Wilms tumor 1, WT33, NPHS4
    Gene ID
    7490
    NCBI Accession
    NP_000369
    UniProt
    P19544
    Pathways
    Tube Formation
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