FASL antibody

Catalog No. ABIN465174

Product Details anti-FASL Antibody

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FASL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Specificity: Human sFas Ligand

Purification: Immunoaffinity purified

Immunogen: CHO cell derived recombinant Human sFas Ligand.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, Neut (0.08 - 0.1 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.75 μg/mL-2.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a AB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of sFasL/Apo1L (10 ng/mL), a concentration of 0.08-0.1 μg/mL of this antibody is required. ELISA: To detect hsFasL/Apo1L by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sFasL/Apo1L (LS-C104462) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsFasL/Apo1L. Western Blot: To detect hsFasL/Apo1L by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsFasL/Apo1L is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: FASLG / FASL / Fas Ligand (FASL Products)

Synonyms: ALPS1B antibody, APT1LG1 antibody, APTL antibody, CD178 antibody, CD95-L antibody, CD95L antibody, FASL antibody, TNFSF6 antibody, fasl antibody, Fas-L antibody, Faslg antibody, Tnfsf6 antibody, gld antibody, Apt1Lg1 antibody, Fasl antibody, FASLG antibody, zgc:162027 antibody, Fas ligand antibody, Fas ligand L homeolog antibody, Fas ligand (TNF superfamily, member 6) antibody, FASLG antibody, faslg.L antibody, Fasl antibody, Faslg antibody, faslg antibody

Background: Name/Gene ID: FASLG
Family: TNF

Synonyms: FASLG, ALPS1B, Apoptosis antigen ligand, APTL, CD95L, CD95 ligand, CD178 antigen, CD95-L, Fas antigen ligand, Fas ligand, FASL, TNFSF6, APT1LG1, CD178

Gene ID: 356

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity