NOV antibody

Catalog No. ABIN465355

Product Details anti-NOV Antibody

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOV antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human NOV

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human NOV.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL-0.50 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hNOV by sandwich ELISA (using 100 μ.l/well antibody solution) a concentration of 0.5-2 μ.g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NOV (LS-C104549) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Alternative Name: CCN3 / NOV (NOV Products)

Synonyms: CCN3 antibody, IBP-9 antibody, IGFBP-9 antibody, IGFBP9 antibody, NOVh antibody, nov-A antibody, xnov antibody, NovH antibody, C130088N23Rik antibody, nephroblastoma overexpressed antibody, nephroblastoma overexpressed L homeolog antibody, nephroblastoma overexpressed gene antibody, NOV antibody, nov.L antibody, Nov antibody

Background: Name/Gene ID: NOV

Synonyms: NOV, CCN3, CCN family member 3, IGFBP9, IBP-9, IGFBP-9, NOVh, IGF-binding protein 9, Nephroblastoma overexpressed, Protein NOV homolog

Gene ID: 4856

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding