TNFSF13 antibody

Catalog No. ABIN465418

Product Details anti-TNFSF13 Antibody

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNFSF13 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Human APRIL

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human APRIL.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human carcinoid tissue. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hAPRIL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human APRIL (LS-C104484) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hAPRIL. Western Blot: To detect hAPRIL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hAPRIL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Alternative Name: TNFSF13 / APRIL (TNFSF13 Products)

Synonyms: APRIL antibody, CD256 antibody, TALL-2 antibody, TALL2 antibody, TRDL-1 antibody, ZTNF2 antibody, 2310026N09Rik antibody, April antibody, Tall2 antibody, Trdl1 antibody, SLAN antibody, ANP32B antibody, april antibody, tnf13 antibody, TNLG7B antibody, TNF superfamily member 13 antibody, tumor necrosis factor (ligand) superfamily, member 13 antibody, acidic leucine-rich nuclear phosphoprotein 32 family member B antibody, TNFSF13 antibody, Tnfsf13 antibody, LOC494442 antibody, tnfsf13 antibody

Background: Name/Gene ID: TNFSF13
Family: TNF

Synonyms: TNFSF13, APRIL, CD256, CD256 antigen, TALL-2, TALL2, ZTNF2, TNF-related death ligand 1, TRDL-1, UNQ383/PRO715

Gene ID: 8741

UniProt: O75888

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response