WNT3A antibody (Biotin)
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- Target See all WNT3A Antibodies
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This WNT3A antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human WNT-3a
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human WNT-3a.
Type of Immunogen: Cell extract - Top Product
- Discover our top product WNT3A Primary Antibody
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- Application Notes
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hWNT-3a by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. To detect hWNT-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human WNT-3a (LS-B8210) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hWNT-3a. Western Blot: To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))
- Alternative Name
- WNT3A (WNT3A Products)
- Synonyms
- WNT3A antibody, Wnt-3a antibody, vt antibody, Zwnt[a] antibody, wnt3 antibody, wnt3 l antibody, wnt3l antibody, wnt[a] antibody, Xwnt-3a antibody, wnt-3a antibody, wnt3a-A antibody, xwnt3a antibody, WNT-3A antibody, WNT3 antibody, wingless-type MMTV integration site family, member 3A antibody, Wnt family member 3A antibody, wingless-type MMTV integration site family member 3A L homeolog antibody, WNT3A antibody, Wnt3a antibody, wnt3a antibody, wnt3a.L antibody
- Background
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Name/Gene ID: WNT3A
Family: WNT
Synonyms: WNT3A, Protein Wnt-3a - Gene ID
- 89780
- UniProt
- P56704
- Pathways
- WNT Signaling, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity
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