Interferon gamma antibody (Biotin)

Catalog No. ABIN465654

Product Details anti-Interferon gamma Antibody (Biotin)

Target: Interferon gamma (IFNG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Interferon gamma antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Specificity: Human IFN-gamma

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IFN-gamma.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), SwELISA, WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect Human IFN-gamma by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma. To detect Human IFN-gamma by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IFN-gamma (LS-B7487) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IFN-gamma. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human IFN-gamma is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Interferon gamma

Target: Interferon gamma (IFNG)

Alternative Name: IFN Gamma / Interferon Gamma (IFNG Products)

Synonyms: IFG antibody, IFI antibody, IFN-g antibody, Ifg antibody, IFNG2 antibody, IFN-gamma antibody, IFN-G antibody, IFNG antibody, IFNgamma antibody, TCRalpha antibody, INF-G antibody, ifng antibody, interferon gamma antibody, interferon, gamma 1-2 antibody, IFNG antibody, Ifng antibody, ifng1-2 antibody

Background: Name/Gene ID: IFNG
Family: Interferon

Synonyms: IFNG, IFG, Immune interferon, IFI, Interferon gamma, IFN-gamma, Interferon, gamma

Gene ID: 3458

UniProt: P01579

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy