BDNF antibody

Catalog No. ABIN465693

The Rabbit Polyclonal anti-BDNF antibody (ABIN465693) specifically detects BDNF in WB, ELISA and Neut. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for BDNF antibody (ABIN465693)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BDNF antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-BDNF Antibody

Specificity: Human BDNF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BDNF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.54 - 0.81 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 μg/mL), a concentration of 0.54-0.81 μg/mL of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile water.

Concentration: Lot specific

Buffer: Lyophilized, PBS,  pH 7.2. Reconstitute with sterile water.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for BDNF

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: Name/Gene ID: BDNF
Family: NGF Beta

Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

Gene ID: 627

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity