GSK3 alpha antibody (AA 203-219)

Catalog No. ABIN470870

The Mouse Monoclonal anti-GSK3 alpha antibody has been validated for WB and ICC. It is suitable to detect GSK3 alpha in samples from Drosophila melanogaster.

Quick Overview for GSK3 alpha antibody (AA 203-219) (ABIN470870)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reactivity: Drosophila melanogaster

Host: Mouse

Clonality: Monoclonal

Conjugate: This GSK3 alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Product Details anti-GSK3 alpha Antibody

Binding Specificity: AA 203-219

Specificity: Recognizes Glycogen Synthase Kinase 3 (GSK3). Due to extremely high conservation among the known enzymes of many different species, should cross-react with nearly all species homologues. Species cross-reactivity: Mouse, rat, S. pombe, Dictyostelium and human.

Predicted Reactivity: Percent identity by BLAST analysis: Drosophila, Mosquito (100%) Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).

Purification: Protein G purified

Immunogen: Synthetic peptide corresponding to aa203-219 (18aa, C-KQLLHGEPNVSYICSRY) derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK-3/ shaggy enzyme (ovalbumin coupled). Percent identity by BLAST analysis: Drosophila, Mosquito (100%), Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Xenopus, Stickleback, Pufferfish, Zebrafish (88%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ICC (10 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot and Immunocytochemistry. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system. Immunocytochemistry: 10 μg/mL. Demonstrated positive immunostaining for GSK3 in L6 cells fixed with acetone. Positive control: J9000: Jurkat Cell.

Comment:

Target Species of Antibody: Drosophila

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Alternative Name: GSK3A / GSK3 Alpha

Background: Name/Gene ID: GSK3A
Subfamily: GSK-3
Family: Protein Kinase

Synonyms: GSK3A, GSK-3 alpha, GSK3 alpha

Gene ID: 2931

UniProt: P49840

Pathways: PI3K-Akt Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effect