AKT 1/2/3 antibody (pTyr315)

Catalog No. ABIN482646

Product Details anti-AKT 1/2/3 Antibody

Target: AKT 1/2/3

Binding Specificity: AA 281-330, pTyr315

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKT 1/2/3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Specificity:

AKT1/2/3 (Phospho-Tyr315/316/312) Antibody detects endogenous levels of AKT1/2/3 only when phosphorylated at Tyr315/316/312.

PhosphorylationH:Y315/316/312 M:Y315/316/312 R:Y315/316/312

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human AKT1/2/3 around the phosphorylation site of Tyr315/316/312.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:20000

Comment:

Unigene-Number: Hs.525622, Hs.631535, Hs.498292 (NCBI Gene Symbol: AKT1)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for AKT 1/2/3

Target: AKT 1/2/3

Alternative Name: AKT1/2/3 (AKT 1/2/3 Products)

Background:

Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT/ RAC-PK-beta, Protein kinase Akt-2, Protein kinase B, beta, PKB beta/ RAC-PK-gamma, Protein kinase Akt-3, Protein kinase B, gamma, PKB gamma, STK-2

NCBI Gene Symbol: AKT1

Molecular Weight: 55 kDa

Gene ID: 207, 208, 10000

OMIM: 164730

UniProt: P31749, P31751, Q9Y243