Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: This antibody was concentrated from Hybridoma (clone HLy-9. 1. 25) supernatant. Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mousesplenocyte.
Flow Cytometry: 10 μg/mLPositive Control: Peripheral blood T cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol
Flow Cytometric Analysis for Floating CellsWe use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS).
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, 7.2 containing 50 % Glycerol without preservatives.
Preservative
Without preservative
Storage
4 °C
Storage Comment
Store the antibody undiluted at 2-8 °C. Shelf life: one year from despatch.
CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9