Bromelain antibody
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- Target
- Bromelain
- Reactivity
- Pineapple
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- Predicted Reactivity
- Ananas comosus
- Characteristics
- Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.
- Purification
- affinity purified
- Immunogen
- native bromelain isolated and purified from Ananas comosus
- Isotype
- IgG
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- Application Notes
- Recommended Dilution: 1 : 1000 - 1 : 100 000 for techniques listed above.
- Comment
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Antibodies have been purified using solid phase affinity chromatography and are stabilized with dextran.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution please add 0.5 mL of sterile destilled water.
- Buffer
- phosphate buffer saline
- Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- Storage
- -20 °C
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- Target
- Bromelain
- Background
- Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
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