CD11b antibody (Activation Site)

Catalog No. ABIN4888495

The Mouse Monoclonal anti-CD11b antibody is suitable to detect CD11b in samples from Human. It has been validated for FACS and IP.

Quick Overview for CD11b antibody (Activation Site) (ABIN4888495)

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD11b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: CBRM1-5

Product Details anti-CD11b Antibody

Binding Specificity: Activation Site

Purpose: Anti-Hu CD11b (activation epitope) Purified

Cross-Reactivity (Details): Human

Characteristics: The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on extracellular part of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and it does not recognize CD11b on non-activated cells.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Information not available

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD11b

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Alternative Name: CD11b

Background: Integrin subunit alpha M,CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18), expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.,Mac-1, Integrin alpha M, ITGAM, CR3A, MO1A, MAC1A

Gene ID: 3684

UniProt: P11215

Pathways: Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation