Histone H3.3 (pSer31) antibody

Details for Product No. ABIN4889664, Supplier: Log in to see
Antigen
  • H3.3A
  • H3F3
  • H3-IX
  • H3-X
  • H3.3B
  • H3F3C
  • H3F3B
  • H3 histone family member 3A
  • H3 histone, family 3B
  • H3 histone, family 3A
  • H3 histone, family 3C L homeolog
  • histone H3.3
  • Histone H3.3
  • histone H3.3B-like
  • H3F3A
  • H3F3B
  • H3f3a
  • h3f3c.L
  • LOC5567559
  • Bm1_29875
  • Bm1_40030
  • PTRG_03457
  • Phum_PHUM492720
  • UREG_07214
  • PAAG_07099
  • PITG_06950
  • PITG_06953
  • PITG_06955
  • LOC100361558
  • LOC100521680
Epitope
pSer31
8
5
3
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
78
29
21
5
5
4
4
3
3
2
1
1
1
1
1
1
1
Host
Rabbit
79
6
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
Application
Dot Blot (DB), Western Blotting (WB)
63
56
23
20
13
12
10
7
2
1
1
1
Options
Supplier
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Immunogen This Histone H3.3 pSer31 antibody was raised against a peptide containing pSer31 of Histone H3.3.
Isotype IgG
Purification Affinity Purified
Antigen
Background Histone H3.3 (H3F3) is a variant of histone H3 that contains a serine (S) to alanine (A) replacement at amino acid position 31. This variant Histone H3.3 has been found to replace conventional histone H3 in nucleosomes of active genes. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. Histone variants differ in amino acid sequence from their core histone counterparts and in some cases have also been shown to have functional differences to the more typical histones. Histone variant H3.3 is the predominant form of histone H3 in non-dividing cells. Phosphorylation of serine 31 of histone variant H3.3 is specific to regions bordering centromeres in metaphase chromosomes. (Reference: Hake et al. (2005) PNAS 102: 6344-6349.)
Molecular Weight 17 kDa
Gene ID 3020
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice

Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Storage -20 °C
Storage Comment Antibodies in solution can be stored at -20 °C for 2 years.
Expiry Date 6 months
Supplier Images
Dot Blot (DB) image for anti-Histone H3.3 (pSer31) antibody (ABIN4889664) Histone H3.3 phospho Ser31 antibody tested by Dot blot. Dot blot analysis was used to...
Western Blotting (WB) image for anti-Histone H3.3 (pSer31) antibody (ABIN4889664) Histone H3.3 phospho Ser31 antibody tested by Western blot. Detection of Histone H3.3...
Product cited in: Chang, Chan, R McGhie, Udugama, Mayne, Collas, Mann, Wong: "CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells." in: Nucleic acids research, Vol. 43, Issue 5, pp. 2603-14, 2015 (PubMed).

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