MYL6 antibody (AA 72-121)
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- Target See all MYL6 Antibodies
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
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Binding Specificity
- AA 72-121
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Reactivity
- Human, Mouse, Rat, Cow, Sheep, Dog, Guinea Pig, Horse, Rabbit, Pig, Zebrafish (Danio rerio), Bat, Monkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MYL6 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human MYL6
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (85%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa72-121 of human MYL6 (P60660, NP_524147). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig, Lizard (100%), Gibbon, Panda, Opossum (91%), Platypus (90%), Hamster, Turkey, Zebra finch, Chicken, Zebrafish (85%), Xenopus (84%).
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product MYL6 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 50 μL sterile ddH2O.
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))
- Alternative Name
- MYL6 (MYL6 Products)
- Synonyms
- ESMLC antibody, LC17 antibody, LC17-GI antibody, LC17-NM antibody, LC17A antibody, LC17B antibody, MLC-3 antibody, MLC1SM antibody, MLC3NM antibody, MLC3SM antibody, zgc:103624 antibody, MLC3nm antibody, Myln antibody, myosin light chain 6 antibody, myosin, light chain 6, alkali, smooth muscle and non-muscle antibody, myosin light chain 6 L homeolog antibody, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle antibody, MYL6 antibody, Myl6 antibody, myl6 antibody, myl6.L antibody
- Background
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Name/Gene ID: MYL6
Synonyms: MYL6, 17 kDa myosin light chain, ESMLC, LC17, LC17B, LC17-GI, LC17A, MLC3NM, MLC1SM, MLC3SM, Myosin light chain A3, Myosin light chain 3, LC17-NM, MLC-3, Myosin light polypeptide 6, Myosin light chain alkali 3 - Gene ID
- 4637
- NCBI Accession
- NP_524147
- UniProt
- P60660
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