BRD3 antibody

Catalog No. ABIN4903072

Product Details anti-BRD3 Antibody

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD3 antibody is un-conjugated

Application: Western Blotting (WB)

Characteristics: Nuclear protein in testis (NUT) is normally confined to the germ cells of the testis and ovary (1,2). NUT midline carcinoma (NMC) is a recently recognized cancer that is defined by the presence of chromosomal rearrangements involving the NUT gene on chromosome 15q14 (3). In most cases the chromosomal translocation occurs between NUT and BRD4 on chromosome 19, resulting in the formation of a BRD4-NUT fusion protein. In the remaining tumors, variant NUT rearrangements are present involving BRD3, a very close homolog of BRD4. BRD4-NUT and BRD3-NUT encode fusion proteins that appear to contribute to carcinogenesis by blocking epithelial cell differentiation. NMCs, which are aggressive and highly lethal carcinomas, are morphologically indistinguishable from other poorly differentiated carcinomas. Given the limited expression of endogenous NUT protein, this antibody can be used to detect NUT fusion proteins in tissues by immunohistochemistry and immunofluorescence (2).

Purification: Affinity purification

Immunogen: Recombinant protein of human BRD3.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for BRD3

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Alternative Name: BRD3 (BRD3 Products)

Synonyms: ORFX antibody, RING3L antibody, 2410084F24Rik antibody, AW060456 antibody, Fsrg2 antibody, RINGL3 antibody, brd3 antibody, fi36b10 antibody, wu:fa13h06 antibody, wu:fc01d09 antibody, wu:fc74a08 antibody, wu:fi04h10 antibody, wu:fi36b10 antibody, BRD3 antibody, ring3l antibody, fa28f05 antibody, wu:fa28f05 antibody, wu:fc56a11 antibody, wu:fj66d12 antibody, zgc:77289 antibody, bromodomain containing 3 antibody, bromodomain containing 3a antibody, bromodomain containing 3 S homeolog antibody, bromodomain-containing protein 3 antibody, bromodomain containing 3b antibody, BRD3 antibody, Brd3 antibody, brd3a antibody, brd3.S antibody, LOC100410652 antibody, LOC100354641 antibody, brd3b antibody

Gene ID: 8019

UniProt: Q15059

Pathways: Chromatin Binding