Histone H4 antibody

Catalog No. ABIN4903928

Product Details anti-Histone H4 Antibody

Target: Histone H4

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Characteristics: The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (1,2). Histone acetylation occurs mainly on the amino-terminal tail domains of histones H2A (Lys5), H2B (Lys5, 12, 15, and 20), H3 (Lys9, 14, 18, 23, 27, and 56), and H4 (Lys5, 8, 12, and 16) and is important for the regulation of histone deposition, transcriptional activation, DNA replication, recombination, and DNA repair (1-3). Hyper-acetylation of the histone tails neutralizes the positive charge of these domains and is believed to weaken histone-DNA and nucleosome-nucleosome interactions, thereby destabilizing chromatin structure and increasing the accessibility of DNA to various DNA-binding proteins (4,5). In addition, acetylation of specific lysine residues creates docking sites for a protein module called the bromodomain, which binds to acetylated lysine residues (6). Many transcription and chromatin regulatory proteins contain bromodomains and may be recruited to gene promoters, in part, through binding of acetylated histone tails. Histone acetylation is mediated by histone acetyltransferases (HATs), such as CBP/p300, GCN5L2, PCAF, and Tip60, which are recruited to genes by DNA-bound protein factors to facilitate transcriptional activation (3). Deacetylation, which is mediated by histone deacetylases (HDAC and sirtuin proteins), reverses the effects of acetylation and generally facilitates transcriptional repression (7,8). Histone H4 lysine 5 is acetylated by multiple HAT proteins. Acetylation by Esa1p in yeast, or Tip60 in mammalian cells, may contribute to both transcriptional activation and DNA repair, including non-homologous end joining and replication-coupled repair (9-12).

Purification: Affinity purification

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:50-1:200, IF 1:20-1:100, IP 1:20-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C.

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products

Synonyms: hist1h4l antibody, H1ft antibody, Hist4 antibody, Hist4h4 antibody, H4 antibody, X04652 antibody, FO108 antibody, H4/n antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4C13 antibody, H4C7 antibody, F8N16.2 antibody, F8N16_2 antibody, histone H4 antibody, BcDNA:RH52884 antibody, CG3379 antibody, Dmel\CG3379 antibody, H4r antibody, His4-88CD antibody, His4R antibody, histone cluster 1, H4a L homeolog antibody, histone cluster 1 H4 family member B antibody, histone cluster 2, H4 antibody, histone cluster 2 H4 family member a antibody, histone H4 antibody, Histone H4 replacement antibody, Histone H4 antibody, hist1h4a.L antibody, Hist1h4b antibody, Hist2h4 antibody, HIST2H4A antibody, H4 antibody, H4C14 antibody, HIS4 antibody, HIST2H4 antibody, His4r antibody, his-38 antibody

Gene ID: 121504

UniProt: P62805