LASS5 antibody (AA 341-354)

Catalog No. ABIN490464

Product Details anti-LASS5 Antibody

Target: LASS5 (LAG1 Homolog, Ceramide Synthase 5 (LASS5))

Binding Specificity: AA 341-354

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Horse, Dog, Monkey, Bat

Host: Goat

Clonality: Polyclonal

Conjugate: This LASS5 antibody is un-conjugated

Application: ELISA

Specificity: Human LASS5. This antibbody is expected to cross-react with the highly similar LASS6.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%) Lizard, Stickleback, Pufferfish, Zebrafish (93%) Hamster, Opossum, Platypus, Xenopus (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RGKVSKDDRSDVES from an internal region of human LASS5 (NP_671723.1, NP_982288.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%), Lizard, Stickleback, Pufferfish, Zebrafish (93%), Hamster, Opossum, Platypus, Xenopus (86%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: Peptide-ELISA (1:4000)

Usage: Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: Preliminary experiments gave an approx. 60  kDa band in lysates of HEK293, HeLa and HepG2 after 0.5 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 45.8  kDa according to NP_671723.1. The 60  kDa band was successfully blocked by incubation with the immunizing peptide.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for LASS5

Target: LASS5 (LAG1 Homolog, Ceramide Synthase 5 (LASS5))

Alternative Name: LASS5 (LASS5 Products)

Synonyms: lass5 antibody, trh4 antibody, LASS5 antibody, Trh4 antibody, 2310081H14Rik antibody, AU045339 antibody, AW544927 antibody, Lass5 antibody, ceramide synthase 5 antibody, ceramide synthase 5 S homeolog antibody, CERS5 antibody, cers5 antibody, cers5.S antibody, Cers5 antibody

Background: Name/Gene ID: CERS5

Synonyms: CERS5, Ceramide synthase 5, LASS5, Trh4

Gene ID: 91012

NCBI Accession: NP_671723, NP_982288

UniProt: Q8N5B7