NCOA1 antibody (N-Term)

Catalog No. ABIN492358

This Mouse Monoclonal antibody specifically detects NCOA1 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for NCOA1 antibody (N-Term) (ABIN492358)

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCOA1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 11F1

Product Details anti-NCOA1 Antibody

Binding Specificity: N-Term

Specificity: AM00146PU-N specifically recognizes the N-terminus of Src in Western blot at 60 kDa.

Characteristics: Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

Purification: Subsequent Subsequent Thiophilic and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: synthetic peptide conjugated to KLHEpitope: N-terminus

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 0.05 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated A431 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated A431 cellsDescription: Cell lysate from untreated A431 cells, epidermoid carcinoma (Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

Target Details for NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Alternative Name: SRC1

Background: Src is the best understood member of a family of 9 tyrosine kinases regulating cellular responses to extracellular stimuli. Src is activated by PDGF and other growth factors that activate transmembrane tyrosine kinase receptors as well as increased intracellular calcium or oxidative stress. Src becomes activated upon dephosphorylation of Tyr 527, leading to a conformational change of the src molecule towards an Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

Gene ID: 6714

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha