Beclin 1 antibody

Catalog No. ABIN492611

Product Details anti-Beclin 1 Antibody

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This Monoclonal antibody recognizes Beclin at 52 kDa in Western-blot application.

Characteristics: Synonyms: BECN1, GT197, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Immunogen: Synthetic peptide conjugated to hemocyanin derived from Beclin sequence.

Clone: 12B4

Isotype: IgG1

Application Details

Application Notes: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Potitive Control: Cell lysate from untreated Neuro 2A (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control: Cell lysate from untreated Neuro2A cells, brain endothelioma (Mouse)Format: Lyophilized cell lysate from serum starved Neuro 2A cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store the antibody in aliquots at -20 °C. Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1 (BECN1 Products)

Synonyms: beclin-1 antibody, atg6 antibody, vps30 antibody, beclin1 antibody, Beclin-1 antibody, becn1 antibody, zgc:73097 antibody, ATG6 antibody, VPS30 antibody, 4921513J16Rik antibody, 5430417M23Rik antibody, Atg6 antibody, beclin 1 antibody, beclin 1, autophagy related antibody, beclin 1 protein antibody, beclin-1 antibody, beclin family protein antibody, beclin 1, autophagy related L homeolog antibody, BECN1 antibody, becn1 antibody, atg6 antibody, CpipJ_CPIJ011087 antibody, PTRG_07825 antibody, SJAG_01876 antibody, Becn1 antibody, becn1.L antibody

Background: Beclin 1 is a ubiquitous coiled-coil protein that plays a central role in autophagy. It interacts with several cofactors (Ambra1, Bif1 , UVRAG) to activate the lipid kinase Vps34, thereby inducing autophagy. In normal conditions, the BH3 domain of Beclin 1 is bound to, and inhibited by, Bcl2 or Bcl-X(L). This interaction can be disrupted by BH3-only proteins to induce autophagy. Nutrient starvation, a potent physiological inducer of autophagy, can stimulate the dissociation of Beclin 1 from its inhibitors, either by activating BH3-only proteins, such as Bad, or by post-translational modifications of Bcl2. Beclin 1 may play a role in anti-viral host defence, and protects against infection by a neurovirulent strain of Sindbis virus. Beclin 1 is also a haploinsufficient tumour suppressor. Genetic studies revealed that Beclin-1 has structural similarity to the yeast autophagy gene, atg6. Beclin-1 is part of a complex with class III PI3 kinase, Vsp34, that is responsible for autophagosome formation.Synonyms: BECN1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197

Molecular Weight: 52 kDa

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy