PAX8 antibody (AA 60-261)

Catalog No. ABIN4949847

Product Details anti-PAX8 Antibody

Target: PAX8 (Paired Box 8 (PAX8))

Binding Specificity: AA 60-261

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX8 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: A human recombinant fragment (aa 60-261) was used as the immunogen for the PAX8 antibody.

Clone: PAX8-1492

Isotype: IgG2a

Application Details

Application Notes: The stated application concentrations are suggested starting points. Titration of the PAX8 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. WB: 0.5-1 μg/mL,IF: 1-2 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the PAX8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for PAX8

Target: PAX8 (Paired Box 8 (PAX8))

Alternative Name: PAX8 (PAX8 Products)

Synonyms: PAX8 antibody, XPax-8 antibody, XPax8 antibody, pax-8 antibody, PAX-8 antibody, Pax-8 antibody, paired box 8 antibody, paired box 8 L homeolog antibody, PAX8 antibody, pax8 antibody, Pax8 antibody, pax8.L antibody

Background: PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins which contain a paired box domain, an octapeptide, and a paired-type homeodomain. The PAX gene family has an important role in the formation of tissues and organs during embryonic development and maintaining the normal function of some cells after birth. The PAX genes give instructions for making proteins that attach themselves to certain areas of DNA. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. PAX8 releases the hormones important for regulating growth, brain development, and metabolism. Also functions in very early stages of kidney organogenesis, the müllerian system, and the thymus. Additionally, PAX8 is expressed in the renal excretory system, epithelial cells of the endocervix, endometrium, ovary, Fallopian tube, seminal vesicle, epididymis, pancreatic islet cells and lymphoid cells. PAX8 and other transcription factors play a role in binding to DNA and regulating the genes that drive thyroid hormone synthesis (Tg, TPO, Slc5a5 and Tshr).

PAX8 (and PAX2) is one of the important regulators of urogenital system morphogenesis. They play a role in the specification of the first renal cells of the embryo and remain essential players throughout development. [Wiki]

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation