TSHR, B Chain antibody

Catalog No. ABIN4949911

Product Details anti-TSHR, B Chain Antibody

Target: TSHR, B Chain (Thyroid Stimulating Hormone Receptor, B Chain (TSHR, B Chain))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TSHR, B Chain antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant human TSHR beta chain protein was used as the immunogen for this TSH Receptor antibody.

Clone: SPM222

Isotype: IgG2a

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TSH Receptor antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the TSH Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for TSHR, B Chain

Target: TSHR, B Chain (Thyroid Stimulating Hormone Receptor, B Chain (TSHR, B Chain))

Alternative Name: Thyroid Stimulating Hormone Receptor / TSHR (beta chain) (TSHR, B Chain Products)

Background: Thyroid-stimulating hormone (TSH, also known as thyrotropin) is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones. TSH is regulated by thyroid hormone (T3) and various retinoid compounds. TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function. The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation. In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits.