The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the VLDL Receptor antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 1-2 μg/mL for 30 min at RT
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Preservative
Azide free
Storage
4 °C,-20 °C
Storage Comment
Store the VLDL Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Target
VLDLR
(Very Low Density Lipoprotein Receptor (VLDLR))
CARMQ1 antibody, CHRMQ1 antibody, VLDLRCH antibody, AA408956 antibody, AI451093 antibody, AW047288 antibody, vldlr antibody, very low density lipoprotein receptor antibody, very low density lipoprotein receptor S homeolog antibody, VLDLR antibody, Vldlr antibody, vldlr.S antibody
Background
Very Low Density Lipoprotein Receptor (VLDLR/VLDL receptor) is a member of the LDL receptor gene family which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDL receptor is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.