CDCP1 antibody

Catalog No. ABIN4950515

Product Details anti-CDCP1 Antibody

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDCP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Antigen affinity

Immunogen: Amino acids R582-T667 of the human protein were used as the immunogen for the CDCP1 antibody.

Isotype: IgG

Application Details

Application Notes: Optimal dilution of the CDCP1 antibody should be determined by the researcher.

1. Required HIER for FFPE samples: Boil the paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to testing.\. Western blot: 0.1-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Storage: -20 °C

Storage Comment: After reconstitution, the CDCP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Target Details for CDCP1

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Alternative Name: CDCP1 (CDCP1 Products)

Synonyms: CD318 antibody, SIMA135 antibody, TRASK antibody, 9030022E12Rik antibody, AA409659 antibody, E030027H19Rik antibody, RGD1305578 antibody, zgc:113071 antibody, CUB domain containing protein 1 antibody, CUB domain containing protein 1a antibody, CDCP1 antibody, Cdcp1 antibody, cdcp1a antibody

Background: CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

UniProt: Q9H5V8