Recombinant Histone H2A, H4 (pSer1) antibody
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- Target
- Histone H2A, H4
- Antibody Type
- Recombinant Antibody
- Binding Specificity
- pSer1
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Reactivity
- All Species
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Characteristics
- This recombinant phospho-Histone H2A/H4 antibody reacts to Histone H2A or Histone H4 phosphorylated at Serine 1. No cross reactivity with other phosphorylated Histones.
- Purification
- Protein A purified from animal origin-free supernatant
- Immunogen
- A phospho-peptide corresponding to phospho-Histone H2A (pSer1) was used as the immunogen for this recombinant phospho-Histone H2A/H4 antibody.
- Clone
- RM216
- Isotype
- IgG
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- Application Notes
- The stated application concentrations are suggested starting points. Titration of the recombinant phospho-Histone H2A/H4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,Immunocytochemistry: 1-2 μg/mL,ELISA: 0.2-1 μg/mL
- Restrictions
- For Research Use only
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- Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store the recombinant phospho-Histone H2A/H4 antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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