E-cadherin antibody (AA 160-189)

Catalog No. ABIN3030243

The Rabbit Polyclonal anti-E-cadherin antibody (ABIN3030243) specifically detects E-cadherin in WB, FACS and ELISA. The antibody is reactive with Human samples.

Quick Overview for E-cadherin antibody (AA 160-189) (ABIN3030243)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA

Product Details anti-E-cadherin Antibody

Binding Specificity: AA 160-189

Purification: Purified

Immunogen: A portion of amino acids 160-189 from the human protein was used as the immunogen for this E Cadherin antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the E Cadherin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the E Cadherin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: E Cadherin

Background: CDH1 is a classical cadherin from the cadherin superfamily. This protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization.

UniProt: P12830

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation