Estrogen Receptor alpha antibody (Isoform 4)

Catalog No. ABIN3029713

This Rabbit Polyclonal antibody specifically detects Estrogen Receptor alpha in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for Estrogen Receptor alpha antibody (Isoform 4) (ABIN3029713)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 283-312, Isoform 4

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 283-312 from the human protein was used as the immunogen for this ER antibody (isoform 4).

Isotype: IgG

Application Details

Application Notes: Titration of the ER antibody (isoform 4) may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the ER antibody (isoform 4) and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: Estrogen Receptor (alpha)

Background: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly