IL12 antibody (AA 168-195)

Catalog No. ABIN3031445

The Rabbit Polyclonal anti-IL12 antibody has been validated for ELISA, WB, FACS and IHC. It is suitable to detect IL12 in samples from Human.

Quick Overview for IL12 antibody (AA 168-195) (ABIN3031445)

Target: IL12 (Interleukin 12 (IL12))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL12 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Product Details anti-IL12 Antibody

Binding Specificity: AA 168-195

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 168-195 from the human protein was used as the immunogen for this IL-12 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the IL-12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the IL-12 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for IL12

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12

Background: IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

UniProt: P29459

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome