PCNA antibody (AA 236-261)

Catalog No. ABIN3032323

This Mouse Monoclonal antibody specifically detects PCNA in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for PCNA antibody (AA 236-261) (ABIN3032323)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 1157CT1-1-3

Product Details anti-PCNA Antibody

Binding Specificity: AA 236-261

Cross-Reactivity (Details): Expected species reactivity: Bovine, Primate, Hamster

Purification: Purified

Immunogen: A portion of amino acids 236-261 from human Proliferating Cell Nuclear Antigen was used as the immunogen for this PCNA antibody.

Isotype: IgG1

Application Details

Application Notes: Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:25,Western blot: 1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA (Proliferating Cell Nuclear Antigen)

Background: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy