Retinoblastoma Protein (Rb) antibody (AA 586-615)

Catalog No. ABIN3032465

The Rabbit Polyclonal anti-Retinoblastoma Protein (Rb) antibody has been validated for WB, ELISA, IHC and IF. It is suitable to detect Retinoblastoma Protein (Rb) in samples from Human.

Quick Overview for Retinoblastoma Protein (Rb) antibody (AA 586-615) (ABIN3032465)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma Protein (Rb) antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Retinoblastoma Protein (Rb) Antibody

Binding Specificity: AA 586-615

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 586-615 from the human protein was used as the immunogen for this Rb antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the Rb antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the Rb antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for Retinoblastoma Protein (Rb)

Target: Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

Alternative Name: Rb (Retinoblastoma)

Background: The retinoblastoma protein is a tumor suppressor protein that is dysfunctional in many types of cancer. One highly studied function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. pRb belongs to the pocket protein family, whose members have a pocket for the functional binding of other proteins. Should an oncogenic protein, such as those produced by cells infected by high-risk types of human papillomaviruses, bind and inactivate pRb, this can lead to cancer. Retinoblastoma (Rb) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral.

UniProt: P06400

Pathways: Mitotic G1-G1/S Phases