RUNX2 antibody (AA 445-474)

Catalog No. ABIN3032500

This Rabbit Polyclonal antibody specifically detects RUNX2 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human.

Quick Overview for RUNX2 antibody (AA 445-474) (ABIN3032500)

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-RUNX2 Antibody

Binding Specificity: AA 445-474

Purification: Antigen affinity purified

Immunogen: A portion of amino acids 445-474 from the human protein was used as the immunogen for this RUNX2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the RUNX2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the RUNX2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: RUNX2

Background: RUNX2 is a transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation. [UniProt]

UniProt: Q13950