Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments in Human Umbilical Cord lysates and in lysates ofcell lines HEK293 and NIH3T3 gave no specific signal but low background (at antibodyconcentration up to 2 μg/mL). Immunohistochemistry: 3-5 μg/mLIn paraffin embedded Human Placenta shows stainng assocated with nuclei in sometrophoblasts and weak cytoplasm staining in cytotrophoblasts.
Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1