KIF11 antibody

Catalog No. ABIN498151

Product Details anti-KIF11 Antibody

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIF11 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Eg5 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for KIF11

Target: KIF11 (Kinesin Family Member 11 (KIF11))

Alternative Name: KIF11 (KIF11 Products)

Synonyms: EG5 antibody, HKSP antibody, KNSL1 antibody, MCLMR antibody, TRIP5 antibody, Eg5 antibody, Kif8 antibody, Kifl1 antibody, Knsl1 antibody, F2H17.19 antibody, F2H17_19 antibody, DDBDRAFT_0189194 antibody, DDBDRAFT_0201556 antibody, DDB_0189194 antibody, DDB_0201556 antibody, XLEg5K1 antibody, XlEg5 antibody, cos2 antibody, costal2 antibody, eg5 antibody, hksp antibody, kif11 antibody, kif11-a antibody, kif11-b antibody, kif11b antibody, knsl1 antibody, trip5 antibody, Cos2 antibody, Costal2 antibody, XLEg5K2 antibody, kif11a antibody, kinesin family member 11 antibody, P-loop containing nucleoside triphosphate hydrolases superfamily protein antibody, kinesin family member 11 S homeolog antibody, kinesin family member 11 L homeolog antibody, KIF11 antibody, Kif11 antibody, AT2G36200 antibody, kif11 antibody, kif11.S antibody, kif11.L antibody

Background: Eukaryotes contain a superfamily of microtubule-based motor proteins comprising Kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. Eg5 (also known as Kinesin-like protein KIF11 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of Eg5 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. Eg5 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). Eg5 is also known to be a member of the thyroid receptor interacting protein (TRIP) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone.Synonyms: EG5, KIF11, KNSL1, Kinesin-like protein 1, Kinesin-like protein KIF11, Kinesin-like spindle protein HKSP, Kinesin-related motor protein Eg5, TRIP-5, TRIP5, Thyroid receptor-interacting protein 5

Molecular Weight: approx. 119 kDa

Gene ID: 3832

NCBI Accession: NP_004514

UniProt: P52732