PDCD4 antibody

Catalog No. ABIN498236

Product Details anti-PDCD4 Antibody

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDCD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of PDCD4 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Alternative Name: PDCD4 (PDCD4 Products)

Synonyms: H731 antibody, D19Ucla1 antibody, Ma3 antibody, Tis antibody, Dug antibody, cb45 antibody, pdcd4 antibody, sb:cb45 antibody, wu:fc84b08 antibody, h731 antibody, MGC69337 antibody, PDCD4 antibody, wu:fa55g03 antibody, wu:fb08a11 antibody, wu:fe01a02 antibody, zgc:66311 antibody, programmed cell death 4 antibody, programmed cell death 4b antibody, programmed cell death 4 L homeolog antibody, programmed cell death 4 (neoplastic transformation inhibitor) antibody, programmed cell death 4a antibody, PDCD4 antibody, Pdcd4 antibody, pdcd4b antibody, pdcd4.L antibody, pdcd4 antibody, pdcd4a antibody

Background: The transformation suppressor gene Pdcd-4 (programmed cell death gene 4) inhibits the tumor-promoter mediated transformation of mouse keratinocytes and is a potential tumor suppressor gene in the development of human lung cancer. Biochemical analysis suggests that the Pdcd-4 protein is involved in protein translation as well as in nuclear events. Pdcd-4 directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Pdcd-4 also suppresses the transactivation of AP-1 responsive promoters by c-Jun, suggesting that the transformation-suppressor activity of Pdcd-4 might be due, at least in part, to the inhibition of c-Jun activity. In addition to affecting c-Jun phosphorylation, Pdcd-4 blocks the recruitment of the coactivator p300 by c-Jun.Synonyms: H731, Neoplastic transformation inhibitor protein, Nuclear antigen H731-like, Programmed cell death protein 4

Molecular Weight: approx. 51 kDa

Gene ID: 27250

NCBI Accession: NP_055271

UniProt: Q53EL6