GTF2H1 antibody

Catalog No. ABIN498311

Product Details anti-GTF2H1 Antibody

Target: GTF2H1 (General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2H1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of TFIIH p62 protein. (region surrounding Ala32)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for GTF2H1

Target: GTF2H1 (General Transcription Factor IIH, Polypeptide 1, 62kDa (GTF2H1))

Alternative Name: GTF2H1 / BTF2 (GTF2H1 Products)

Synonyms: 62kDa antibody, AW743425 antibody, AW822074 antibody, BTF2 p62 antibody, C77871 antibody, p62 antibody, GTF2H1 antibody, BTF2 antibody, P62 antibody, TFB1 antibody, TFIIH antibody, gtf2h1 antibody, MGC53309 antibody, btf2 antibody, tfiih antibody, CG8151 antibody, Dmel\\CG8151 antibody, Dmp62 antibody, TfBl antibody, l(2)06949 antibody, l(2)06950 antibody, zgc:92126 antibody, general transcription factor IIH subunit 1 antibody, general transcription factor II H, polypeptide 1 antibody, general transcription factor IIH subunit 1 L homeolog antibody, Transcription factor B1 antibody, general transcription factor IIH, polypeptide 1 antibody, GTF2H1 antibody, Gtf2h1 antibody, gtf2h1.L antibody, gtf2h1 antibody, Tfb1 antibody

Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription actors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFII. TFIIH (or BTF2) is a multisubunit transcription/DNA repair factor that possesses several enzymatic activities. The core of TFIIH is composed of five subunits, designated p89 (XPB or ERCC3), p62, p52, p44 and p34. Additional subunits of the TFIIH complex are p80 (XPD or ERCC2) and the ternary kinase complex composed of Cdk7, cyclin H and MAT1. Both p89 and p80 have ATP-dependent helicase activity. The p62, p52 and p44 subunits have been shown to be involved in nucleotide excision repair.Synonyms: BTF2-p62, Basic transcription factor 2 62 kDa subunit, General transcription factor IIH polypeptide 1, General transcription factor IIH subunit 1, TFIIH, TFIIH basal transcription factor complex p62 subunit

Molecular Weight: approx. 57 kDa

Gene ID: 2965

NCBI Accession: NP_001135779

UniProt: P32780

Pathways: DNA Damage Repair