CD3 antibody
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- Target See all CD3 Antibodies
- CD3
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of CD3-epsilon protein. (region surrounding Asp71)
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Purification
- Affinity chromatography
- Purity
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product CD3 Primary Antibody
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- Application Notes
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD3
- Alternative Name
- CD3 (CD3 Products)
- Synonyms
- CD3 antibody, AI504783 antibody, CD3epsilon antibody, T3e antibody, cd3-gamma antibody, cd3g antibody, cd3g-a antibody, cd3g/d antibody, cd3gd antibody, t3g antibody, CD3e molecule antibody, CD3d molecule antibody, CD3 antigen, epsilon polypeptide antibody, CD3g molecule S homeolog antibody, CD3E antibody, CD3D antibody, Cd3e antibody, cd3g.S antibody
- Background
- The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (Cluster of Differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (gamma-epsilon), a heterodimer of delta and epsilon chains (delta-epsilon) and a homodimer of two zeta chains (zeta-zeta) or a heterodimer of zeta and eta chains (zeta-eta). The zeta and eta chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the zeta chain contains three consecutive copies of the same motif.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
- Molecular Weight
- approx. 28 kDa
- Gene ID
- 916
- NCBI Accession
- NP_000724
- UniProt
- P07766
- Pathways
- TCR Signaling, Ubiquitin Proteasome Pathway
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