HSF1 antibody

Catalog No. ABIN498574

Product Details anti-HSF1 Antibody

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of HSF1 protein. (region surrounding Val297)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: Heat Shock Factor 1 / HSF1 (HSF1 Products)

Synonyms: hsf1 antibody, Hsf antibody, xhsf1 antibody, AA960185 antibody, HSTF1 antibody, HSF1 antibody, HSF antibody, HSF1c antibody, hsf1a antibody, hsf1b antibody, zHSF1a antibody, zHSF1b antibody, hstf1 antibody, heat shock factor antibody, Heat Shock Factor antibody, heat shock factor 1 antibody, heat shock transcription factor 1 antibody, heat shock transcription factor 1 S homeolog antibody, hsf1 antibody, hsf-1 antibody, Hsf1 antibody, HSF1 antibody, hsf1.S antibody

Background: Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1

Molecular Weight: approx. 82 kDa

Gene ID: 3297

NCBI Accession: NP_005517

UniProt: Q00613