DOK2 antibody (pTyr299)

Catalog No. ABIN498759

Product Details anti-DOK2 Antibody

Target: DOK2

Binding Specificity: pTyr299

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of Dok-2 protein when phosphorylated at Tyr299.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DOK2

Target: DOK2

Alternative Name: DOK2 / p56 Dok2 (DOK2 Products)

Synonyms: DokR antibody, Frip antibody, Dok-2 antibody, p56DOK antibody, p56dok-2 antibody, docking protein 2 antibody, DOK2 antibody, Dok2 antibody

Background: Dok-1 associates with the Ras GTPase activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that p62 Dok-1 is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9,22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, is a member of a class of Synonyms: Docking protein 2, Downstream of tyrosine kinase 2, p56(dok-2), p56Dok-2

Molecular Weight: approx. 56 kDa

Gene ID: 9046

NCBI Accession: NP_003965

UniProt: O60496