ATM antibody (pSer1981)

Catalog No. ABIN498773

The Rabbit Polyclonal anti-ATM antibody is suitable to detect ATM in samples from Human. It has been validated for IHC (p).

Quick Overview for ATM antibody (pSer1981) (ABIN498773)

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATM antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATM Antibody

Binding Specificity: pSer1981

Specificity: This antibody detects endogenous levels of p-ATM protein only when phosphorylated at Ser1981.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATM

Target: ATM (Ataxia Telangiectasia Mutated (ATM))

Alternative Name: ATM

Background: The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4- kinases and the second group comprises the PIK-related kinases. The PIKrelated kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy-termini that is not present in the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and they display delays in p53 induction. Cultured cells respond to breaks in double stranded DNA by immediate activation of ATM through autophosphorylation on Serine 1981. Most human tissues, however, contain the nonphosphorylated, inactive form of Atm.Synonyms: A-T mutated, ATDC, Ataxia telangiectasia mutated, Serine-protein kinase ATM, TEL1, TELO1

Molecular Weight: approx. 350 kDa

Gene ID: 472

NCBI Accession: NP_000042

UniProt: Q13315

Pathways: p53 Signaling, Apoptosis, DNA Damage Repair, Inositol Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus