PDGFRB antibody (pTyr751)

Catalog No. ABIN498798

Product Details anti-PDGFRB Antibody

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Binding Specificity: pTyr751

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDGFRB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of PDGFR beta protein only when phosphorylated at Tyr751.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Alternative Name: CD140b / PDGFRB (PDGFRB Products)

Synonyms: AI528809 antibody, CD140b antibody, PDGFR-1 antibody, Pdgfr antibody, CD140B antibody, IBGC4 antibody, JTK12 antibody, PDGFR antibody, PDGFR1 antibody, platelet derived growth factor receptor, beta polypeptide antibody, platelet derived growth factor receptor beta antibody, Pdgfrb antibody, PDGFRB antibody

Background: Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and gliaderived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-alpha and -beta, which are endowed with protein tyrosine kinase domains. PDGFR-alpha can bind to both A and B subunits of PDGF, while PDGFR-beta can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two alpha receptors, PDGF-AB induces dimerization of alpha-alpha and alpha-beta, and PDGF-BB induces the formation of three types of dimers, alpha-alpha, alpha-beta and beta-beta. Translocation of the PDGFR-beta gene with the TEL gene is linked with chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.Synonyms: Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, PDGF Receptor beta, PDGF-R-beta

Molecular Weight: approx. 124, 190 kDa

Gene ID: 5159

NCBI Accession: NP_002600

UniProt: P09619

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling