eIF4EBP1 antibody (C-Term)

Catalog No. ABIN499179

This anti-eIF4EBP1 antibody is a Rabbit Polyclonal antibody detecting eIF4EBP1 in WB, IF and EIA. Suitable for Human and Mouse.

Quick Overview for eIF4EBP1 antibody (C-Term) (ABIN499179)

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This eIF4EBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-eIF4EBP1 Antibody

Binding Specificity: C-Term

Specificity: 4E-BP1 antibody was raised against a 14 amino acid peptide from near the carboxy-terminus of human 4E-BP1.

Purification: Affinity chromatography purified via peptide column

Immunogen: A 14 amino acid peptide from near the carboxy-terminus of Human 4E-BP1

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 4E-BP1 antibody can be used for the detection of 4E-BP1 at 2.5 - 5 μg/mL. Immunofluorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for eIF4EBP1

Target: eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))

Alternative Name: EIF4EBP1 / 4E-BP1

Background: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms (reviewed in 1). One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 2). One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5'-UTRs (1). Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 3), at least in part by inhibiting the activity of TOR towards 4E-BP1.Synonyms: Eukaryotic translation initiation factor 4E-binding protein 1, PHAS-1, PHAS-I, PHAS1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1

Gene ID: 1978

NCBI Accession: NP_004086

UniProt: Q13541

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling