EZH1 antibody (AA 471-510) (Alexa Fluor 680)

Catalog No. ABIN5002238

Product Details anti-EZH1 Antibody (Alexa Fluor 680)

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Binding Specificity: AA 471-510

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EZH1 antibody is conjugated to Alexa Fluor 680

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Chicken,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human EZH1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for EZH1

Target: EZH1 (Enhancer of Zeste Homolog 1 (EZH1))

Alternative Name: EZH1 (EZH1 Products)

Synonyms: KMT6B antibody, si:ch211-250i3.1 antibody, zgc:136829 antibody, AW557915 antibody, mKIAA0388 antibody, EZH1 antibody, enhancer of zeste 1 polycomb repressive complex 2 subunit antibody, EZH1 antibody, ezh1 antibody, Ezh1 antibody

Background:

Synonyms: Enhancer of zeste homolog 1Drosophila, Enhancer of zeste homolog 1, ENX-2, ENX2, EZH1, EZH1: enhancer of zeste homolog 1 Drosophila, EZH1_HUMAN, Histone-lysine N-methyltransferase EZH1, KIAA0388.

Background: Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH1-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. Sequence similarities Belongs to the histone-lysine methyltransferase family. EZ subfamily.Contains 1 SET domain.

Gene ID: 2145