GFP antibody (AA 25-120) (AbBy Fluor® 750)

Catalog No. ABIN5003408

This anti-GFP antibody is a Rabbit Polyclonal antibody detecting GFP in WB, IF (p) and IF (cc). Suitable for Aequorea victoria and Aequorea coerulescens (Belt Jellyfish).

Quick Overview for GFP antibody (AA 25-120) (AbBy Fluor® 750) (ABIN5003408)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria, Aequorea coerulescens (Belt Jellyfish)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to AbBy Fluor® 750

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Product Details anti-GFP Antibody (AbBy Fluor® 750)

Binding Specificity: AA 25-120

Cross-Reactivity: Pig, Tag

Cross-Reactivity (Details): Aequorea victoria, Aequorea coerulescens (belt jellyfish)

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from jellyfish Green Fluorecent protein

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background:

Synonyms: Green fluorescence protein, GRP, GFP TAG, GFP-TAG.

Background: Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

UniProt: P42212