PLEKHM3 antibody (C-Term)

Catalog No. ABIN500513

Product Details anti-PLEKHM3 Antibody

Target: PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLEKHM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: Peptide affinity chromatography

Immunogen: PLEKHM3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human PLEKHM3.

Isotype: IgG

Application Details

Application Notes: ELISA: Western blot: 1 - 2 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for PLEKHM3

Target: PLEKHM3 (Pleckstrin Homology Domain Containing, Family M, Member 3 (PLEKHM3))

Alternative Name: PLEKHM3 (PLEKHM3 Products)

Synonyms: PLEKHM1L antibody, DAPR antibody, 9430067K14Rik antibody, A230102O09Rik antibody, AI449570 antibody, Plekhm1l antibody, Dapr antibody, RGD1307151 antibody, pleckstrin homology domain containing, family M, member 3 antibody, pleckstrin homology domain containing M3 antibody, pleckstrin homology domain containing M3 L homeolog antibody, PLEKHM3 antibody, Plekhm3 antibody, plekhm3.L antibody

Background: PLEKHM3, also known as DAPR, is a member of the M family of Pleckstrin homology domain-containing proteins. PLEKHM3 was initially identified through chromatin immunoprecipitation and CpG microarray analysis examining proteins regulated by myocyte-enhancing factor 2. In C2C12 myoblast cells, PLEKHM3 binds to the PI3K signaling member protein kinase B in the cytosol prior to differentiation into myotubes. Following the initiation of differentiation, PLEKHM3 was also found in membrane fractions. Knockdown of PLEKHM3 expression by RNAi resulted in the inhibition of myotube formation, suggesting that PLEKHM3 is a key component required by myoblasts for orchestrating their differentiation during myogenesis.Synonyms: PH domain-containing family M member 3, PLEKHM1L, Pleckstrin homology domain-containing family M member 3

Gene ID: 389072

UniProt: Q6ZWE6