Ubiquitin antibody (PE)
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- Target See all Ubiquitin Antibodies
- Ubiquitin
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Ubiquitin antibody is conjugated to PE
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.
- Purification
- Gel filtration
- Immunogen
- Ubiquitin conjugated lysozyme
- Clone
- FK1
- Isotype
- IgM
- Top Product
- Discover our top product Ubiquitin Primary Antibody
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- Application Notes
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- WB (1:10000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- Ubiquitin
- Alternative Name
- Ubiquitin (Ubiquitin Products)
- Synonyms
- CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
- Background
- Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.
- Gene ID
- 7314
- UniProt
- P0CG47
- Pathways
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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