NLRP3 antibody (AA 150-385)

Catalog No. ABIN5027384

This anti-NLRP3 antibody is a Mouse Monoclonal antibody detecting NLRP3 in WB and IHC. Suitable for Human and Mouse.

Quick Overview for NLRP3 antibody (AA 150-385) (ABIN5027384)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: ABM2D48

Product Details anti-NLRP3 Antibody

Binding Specificity: AA 150-385

Purification: Protein G Chromatography

Immunogen: A partial length recombinant NALP3 protein (amino acids 150-385) was used as the immunogen for this antibody.

Isotype: IgG2b kappa

Application Details

Application Notes: WB: 6-8 μg/mL, IHC: 5 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 0.05 % BSA, PH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: NALP3

Background: NALP3 (NACHT, LRR and PYD domains-containing protein 3), otherwise known as cryopyrin, is a member of the NLR (Nod-like-receptors) family. This is a pyrin-like protein, having N-terminal CARD or pyrin domains needed for homotypic protein protein interaction, an intermediate nucleotide binding self oligomerization NACHT domain and a C-terminal domain containing leucine rich repeats (LRRs). NALP3 is a key component of innate immunity and its expression is restricted to immune cells and chondrocytes. It interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kB signaling, and it plays an important role in the regulation of inflammation, the immune response, and apoptosis.

Molecular Weight: 120 kDa

Gene ID: 114548

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome