IGF1 antibody (Atto 488)

Catalog No. ABIN5066643

This Rabbit Polyclonal antibody specifically detects IGF1 in WB, IHC, ICC and IF. It exhibits reactivity toward Human.

Quick Overview for IGF1 antibody (Atto 488) (ABIN5066643)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to Atto 488

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-IGF1 Antibody (Atto 488)

Specificity: Detects ~25 kDa.

Cross-Reactivity: Human, Mouse

Purification: Peptide Affinity Purified

Immunogen: Synthetic peptide from the mid-protein of Human IGF-1

Isotype: IgG

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5066643 was sufficient for detection of IGF-1 in 15 μg of mouse liver cell lysates by ECL immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF-1

Gene ID: 3479

NCBI Accession: NP_000609

UniProt: P05019

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation