O-GlcNAc antibody (PE)
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- Target
- O-GlcNAc
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- PE
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
- Specificity
- Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins.
- Purification
- PEG Purified
- Immunogen
- Synthetic beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
- Clone
- 9H6
- Isotype
- IgM
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- IHC (1:25)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5067143 was sufficient for detection of O-GlcNAc in 2 μg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- O-GlcNAc
- Target Type
- Viral Protein
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