7-Ketocholesterol (7-KC) antibody (HRP)
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- Target
- 7-Ketocholesterol (7-KC)
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- HRP
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Application
- Immunofluorescence (IF), Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- Specificity
- Specific for 7-ketocholesterol modified proteins.
- Purification
- Protein G Purified
- Immunogen
- Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).
- Clone
- 3F7
- Isotype
- IgG3
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:50)
- ELISA (1:1000)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN5067338 was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Conjugated antibodies should be stored at 4°C
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- Target
- 7-Ketocholesterol (7-KC)
- Alternative Name
- 7-Ketocholesterol
- Target Type
- Hormone
- Background
- Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. 7-ketocholesterol (7-KC) is a biomarker of lipoprotein cholesterol oxidation.
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