Methylglyoxal (MG) antibody (Biotin)

Catalog No. ABIN5067390

Product Details

Target: Methylglyoxal (MG)

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC)

Specificity: Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, MDA, 4-HHE, 4-HNE, or Crotonaldehyde modified proteins.

Purification: Protein G Purified

Immunogen: Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Clone: 9E7

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:50)
• FACS (1:50)
• FCM (1:50)
• ELISA (1:1000)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN5067390 was sufficient for detection of Methylglyoxal in 0.5 μg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Conjugated antibodies should be stored at 4°C

Target Details

Target: Methylglyoxal (MG)

Alternative Name: Methylglyoxal

Target Type: Chemical

Background: Lipid peroxidation occurs when oxidizing agents attack carbon-carbon double bonds found in unsaturated lipids. In addition to membrane degradation, oxidation end-products have been found to damage cell viability through their mutagenic and toxic properties. These downstream functional consequences facilitate the development of disease and premature aging. Methylglyoxal is an alpha-oxoaldehyde generated from lipid peroxidation that reacts with proteins to form adducts(1). It reacts with free amino acids and protein residues to generate advanced glycation endproducts (AGEs) and crosslinks DNA polymerase and substrate DNA to severely inhibit DNA replication (2).